rabbit polyclonal antibodies against zeb2  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland

doi: 10.1371/journal.pone.0209497

Figure Lengend Snippet: (A) Invasive micropapillary areas characterized by a decrease of E-cadherin expression. Some cells show high-intensity cytoplasmic membrane E-cadherin expression (arrow). This case was classified as 1+. Advance HRP peroxidase system and anti-E-cadherin; counterstained with Harris’s hematoxylin; scale bar, 30 μm. (B) The invasive micropapillary area is exhibiting neoplastic cells with the nuclear expression for ZEB2 (arrow). The inset shows details of the staining. Advance HRP peroxidase system and anti-ZEB2; counterstained with Harris’s hematoxylin; scale bar, 30 μm.

Article Snippet: The sections were next incubated with a mouse monoclonal antibody against E-cadherin (1:80, clone 4A2C7, Invitrogen) and a rabbit polyclonal antibody against ZEB2 (1:200, polyclonal, Sigma-Aldrich), overnight at 4°C, and then rinsed 3 times for 5 min in PBS.

Techniques: Expressing, Staining

Journal: PLoS ONE

Article Title: The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland

doi: 10.1371/journal.pone.0209497

Figure Lengend Snippet: Confocal immunofluorescence image of the cytoplasmic membrane E-cadherin (green) (A), nuclear ZEB2 (red) (B) and nuclear staining with Hoechst (blue) (C). Note that most of the neoplastic cells present both, positivity for ZEB2 and negativity for E-cadherin. Some neoplastic cells reveal concomitantly positivity for ZEB2 and weak intensity E-cadherin expression. Nuclear localization of ZEB2 was confirmed for the merged image (D) that demonstrates the co-localisation of ZEB2 with Hoechst. Images are representative of what was observed in 10 fields of an IMPC case. Scale Bar = 20 μm.

Article Snippet: The sections were next incubated with a mouse monoclonal antibody against E-cadherin (1:80, clone 4A2C7, Invitrogen) and a rabbit polyclonal antibody against ZEB2 (1:200, polyclonal, Sigma-Aldrich), overnight at 4°C, and then rinsed 3 times for 5 min in PBS.

Techniques: Immunofluorescence, Staining, Expressing

Journal: PLoS ONE

Article Title: The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland

doi: 10.1371/journal.pone.0209497

Figure Lengend Snippet: Number of neoplastic epithelial cells stained for E-cadherin (ECAD) and ZEB2 in immunofluorescence analysis performed in invasive areas of IMPC of the canine mammary gland.

Article Snippet: The sections were next incubated with a mouse monoclonal antibody against E-cadherin (1:80, clone 4A2C7, Invitrogen) and a rabbit polyclonal antibody against ZEB2 (1:200, polyclonal, Sigma-Aldrich), overnight at 4°C, and then rinsed 3 times for 5 min in PBS.

Techniques: Staining, Immunofluorescence

Journal: PLoS ONE

Article Title: The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland

doi: 10.1371/journal.pone.0209497

Figure Lengend Snippet: Number of neoplastic epithelial cells stained for E-cadherin (ECAD) and ZEB2 concomitantly (ECAD + ZEB2 + ) and their distribution in ECAD Strong ZEB2 + and ECAD Weak ZEB2 + groups based on the staining intensity for E-cadherin through immunofluorescence in invasive areas of IMPC of the canine mammary gland.

Article Snippet: The sections were next incubated with a mouse monoclonal antibody against E-cadherin (1:80, clone 4A2C7, Invitrogen) and a rabbit polyclonal antibody against ZEB2 (1:200, polyclonal, Sigma-Aldrich), overnight at 4°C, and then rinsed 3 times for 5 min in PBS.

Techniques: Staining, Immunofluorescence

Journal: PLoS ONE

Article Title: The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland

doi: 10.1371/journal.pone.0209497

Figure Lengend Snippet: (A) Invasive micropapillary area of a IMPC classified as 4+ for E-cadherin. The case reveals >10 dots/cell and more than 10% positive cells with dot clusters. RNAscope (Advanced Cell Diagnostics) approach; counterstained with Gill´s hematoxylin; scale bar, 30 μm. (B) Invasive micropapillary area of a IMPC classified as 2+ for ZEB2 . The neoplastic cells show 4–10 dots/cell and very few dot clusters. The inset shows details of the staining. RNAscope (Advanced Cell Diagnostics) approach; counterstained with Gill´s hematoxylin; scale bar, 30 μm.

Article Snippet: The sections were next incubated with a mouse monoclonal antibody against E-cadherin (1:80, clone 4A2C7, Invitrogen) and a rabbit polyclonal antibody against ZEB2 (1:200, polyclonal, Sigma-Aldrich), overnight at 4°C, and then rinsed 3 times for 5 min in PBS.

Techniques: Staining

Journal: PLoS ONE

Article Title: The investigation of transcriptional repression mediated by ZEB2 in canine invasive micropapillary carcinoma in mammary gland

doi: 10.1371/journal.pone.0209497

Figure Lengend Snippet: Immunohistochemical (IHC) and RNA In Situ Hybridization (ISH) staining for ZEB2 and E-cadherin ( CDH1 ) in invasive areas of IMPC of the canine mammary gland.

Article Snippet: The sections were next incubated with a mouse monoclonal antibody against E-cadherin (1:80, clone 4A2C7, Invitrogen) and a rabbit polyclonal antibody against ZEB2 (1:200, polyclonal, Sigma-Aldrich), overnight at 4°C, and then rinsed 3 times for 5 min in PBS.

Techniques: Immunohistochemical staining, RNA In Situ Hybridization, Staining